METTL3 inhibits inflammation of retinal pigment epithelium cells by regulating NR2F1 in an m⁶A-dependent manner.

ABSTRACT: N⁶-metyladenosine (m⁶A) RNA methylation has been proven to be involved in diverse biological processes, but its potential roles in the development of lipopolysaccharide (LPS) induced retinal pigment epithelium (RPE) inflammation have not been revealed. In this study, we explored the effects and underlying mechanisms of methyltransferase-like 3 (METTL3) in LPS stimulated RPE cells. Proliferation of METTL3-silenced RPE cells was examined by Cell counting kit-8 (CCK8) and 5-Ethynyl-2´-Deoxyuridine (Edu). Expression of tight junction proteins ZO-1 and Occludin, and secretion of inflammatory factors interleukins (IL)-1, 6 and 8 were detected by Western blotting or Enzyme-linked immunosorbent assay (ELISA). RNA sequencing and methylated RNA immunoprecipitation (MeRIP) seque

SUBMITTER: Meng J 

PROVIDER: S-EPMC9351451 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature