Stochastic particle unbinding modulates growth dynamics and size of transcription factor condensates in living cells.

ABSTRACT: Liquid-liquid phase separation (LLPS) is emerging as a key physical principle for biological organization inside living cells, forming condensates that play important regulatory roles. Inside living nuclei, transcription factor (TF) condensates regulate transcriptional initiation and amplify the transcriptional output of expressed genes. However, the biophysical parameters controlling TF condensation are still poorly understood. Here we applied a battery of single-molecule imaging, theory, and simulations to investigate the physical properties of TF condensates of the progesterone receptor (PR) in living cells. Analysis of individual PR trajectories at different ligand concentrations showed marked signatures of a ligand-tunable LLPS process. Using a machine learning architecture, we found

SUBMITTER: Munoz-Gil G 

PROVIDER: S-EPMC9351496 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature