Correlation between the binding affinity and the conformational entropy of nanobody SARS-CoV-2 spike protein complexes.

ABSTRACT: Camelid single-domain antibodies, also known as nanobodies, can be readily isolated from naïve libraries for specific targets but often bind too weakly to their targets to be immediately useful. Laboratory-based genetic engineering methods to enhance their affinity, termed maturation, can deliver useful reagents for different areas of biology and potentially medicine. Using the receptor binding domain (RBD) of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein and a naïve library, we generated closely related nanobodies with micromolar to nanomolar binding affinities. By analyzing the structure-activity relationship using X-ray crystallography, cryoelectron microscopy, and biophysical methods, we observed that higher conformational entropy losses in the formatio

SUBMITTER: Mikolajek H 

PROVIDER: S-EPMC9351521 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature