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Dual functions of discoidinolysin, a cholesterol-dependent cytolysin with N-terminal discoidin domain produced from Streptococcus mitis strain Nm-76.


ABSTRACT:

Background

Some strains of Streptococcus mitis exhibit β-hemolysis due to the β-hemolytic activity of cholesterol-dependent cytolysin (CDC). Recently, a gene encoding an atypical lectinolysin-related CDC was found in S. mitis strain Nm-76. However, the product of this gene remains uncharacterized. We aimed to characterize this atypical CDC and its molecular functions and contribution to the pathogenicity of S. mitis strain Nm-76.

Methods

Phylogenetic analysis of the CDC gene was conducted based on the web-deposited information. The molecular characteristics of CDC were investigated using a gene-deletion mutant strain and recombinant proteins expressed in Escherichia coli.

Results

The gene encoding CDC found in Nm-76 and its homolog are distributed among many S. mitis strains. This CDC is phylogenetically different from other previously characterized CDCs, such as S. mitis-derived human platelet aggregation factor (Sm-hPAF)/lectinolysin and mitilysin. Because this CDC possesses an additional N-terminal domain, including a discoidin motif, it was termed discoidinolysin (DLY). In addition to the preferential lysis of human cells, DLY displayed N-terminal domain-dependent facilitation of human erythrocyte aggregation and intercellular associations between human cells.

Conclusion

DLY functions as a hemolysin/cytolysin and erythrocyte aggregation/intercellular association molecule. This dual-function DLY could be an additional virulence factor in S. mitis.

SUBMITTER: Tabata A 

PROVIDER: S-EPMC9351568 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Dual functions of discoidinolysin, a cholesterol-dependent cytolysin with N-terminal discoidin domain produced from <i>Streptococcus mitis</i> strain Nm-76.

Tabata Atsushi A   Matsumoto Airi A   Fujimoto Ai A   Ohkura Kazuto K   Ikeda Takuya T   Oda Hiroki H   Yokohata Shuto S   Kobayashi Miho M   Tomoyasu Toshifumi T   Takao Ayuko A   Ohkuni Hisashi H   Nagamune Hideaki H  

Journal of oral microbiology 20220801 1


<h4>Background</h4>Some strains of <i>Streptococcus mitis</i> exhibit β-hemolysis due to the β-hemolytic activity of cholesterol-dependent cytolysin (CDC). Recently, a gene encoding an atypical lectinolysin-related CDC was found in <i>S. mitis</i> strain Nm-76. However, the product of this gene remains uncharacterized. We aimed to characterize this atypical CDC and its molecular functions and contribution to the pathogenicity of <i>S. mitis</i> strain Nm-76.<h4>Methods</h4>Phylogenetic analysis  ...[more]

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