Primary breast cancer biomarkers based on glycosylation and extracellular vesicles detected from human serum.

ABSTRACT:

Background

Breast cancer is a very common cancer that can be severe if not discovered early. The current tools to detect breast cancer need improvement. Cancer has a universal tendency to affect glycosylation. The glycosylation of circulating extracellular vesicle-associated glycoproteins, and mucins may offer targets for detection methods and have been only explored in a limited capacity.

Aim

Our aim was to develop an approach to detect the aberrant glycosylation of mucins and extracellular vesicle-associated glycoproteins from human sera using fluorescent nanoparticles, and preliminarily evaluate this approach for the differential diagnosis of breast cancer.

Methods and results

The assay involved immobilizing glycosylated antigens using monoclonal antibodies and the

SUBMITTER: Terava J 

PROVIDER: S-EPMC9351655 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature