ATF4 Protects the Heart From Failure by Antagonizing Oxidative Stress.

ABSTRACT:

Background

Cellular redox control is maintained by generation of reactive oxygen/nitrogen species balanced by activation of antioxidative pathways. Disruption of redox balance leads to oxidative stress, a central causative event in numerous diseases including heart failure. Redox control in the heart exposed to hemodynamic stress, however, remains to be fully elucidated.

Methods

Pressure overload was triggered by transverse aortic constriction in mice. Transcriptomic and metabolomic regulations were evaluated by RNA-sequencing and metabolomics, respectively. Stable isotope tracer labeling experiments were conducted to determine metabolic flux in vitro. Neonatal rat ventricular myocytes and H9c2 cells were used to examine molecular mechanisms.

Results

We show that prod

SUBMITTER: Wang X 

PROVIDER: S-EPMC9351829 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature