Assessing the diagnostic performance of a novel RT-PCR fluorescence method for the detection of human plasmodium species.

ABSTRACT:

Background

Malaria elimination effort is hampered not only by the lack of effective medication but also due to the lack of sensitive diagnostic tools to detect infections with low levels of parasitemia. Therefore, more sensitive and specific high-throughput molecular diagnostic approaches are needed for accurate malaria diagnosis.

Methods

In the present study, the performance of a novel single-tube MC004 real-time polymerase chain reaction (PCR) assay (MRC-Holland, Amsterdam, the Netherlands) was assessed for the detection of infection and discrimination of Plasmodium species. Blood samples (n = 150) were collected from malaria suspected patients at Adama malaria diagnosis and treatment centre, Adama, central Ethiopia. The positive predictive value (PPV), negative predictive

SUBMITTER: Beyene MB 

PROVIDER: S-EPMC9352105 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature