Unbiased Data Analysis for the Parameterization of Fast Translocation Events through Nanopores.

ABSTRACT: Single-molecule nanopore electrophysiology is an emerging technique for the detection of analytes in aqueous solutions with high sensitivity. These detectors have proven applicable for the enzyme-assisted sequencing of oligonucleotides. There has recently been an increased interest in the use of nanopores for the fingerprinting of peptides and proteins, referred to as single-molecule nanopore spectrometry. However, the analysis of the resulting electrophysiology traces remains complicated due to the fast unassisted translocation of such analytes, usually in the order of micro- to milliseconds, and the small ion current signal produced (in the picoampere range). Here, we present the application of a generalized normal distribution function (gNDF) for the characterization of short-lived ion

SUBMITTER: Lucas FLR 

PROVIDER: S-EPMC9352258 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature