Discovery and Characterization of a Novel Allosteric Small-Molecule Inhibitor of NADP⁺-Dependent Malic Enzyme 1.

ABSTRACT: NADP⁺-dependent malic enzyme 1 (ME1) decarboxylates malate to form pyruvate and NADPH in the cytoplasm, where it mediates diverse biological functions related to the generation of lipids and other cellular building blocks. As such, ME1 has been implicated in the progression of cancers and has received attention as a promising drug target. Here we report the identification of a novel small-molecule inhibitor of ME1, designated AS1134900. AS1134900 is highly selective for ME1 compared with ME2 and uncompetitively inhibits ME1 activity in the presence of its substrates NADP⁺ and malate. In addition, X-ray crystal structure analysis of the enzyme-inhibitor complex revealed that AS1134900 binds outside the ME1 active site in a novel allosteric site. Structural comparison o

SUBMITTER: Yoshida T 

PROVIDER: S-EPMC9352307 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature