Impaired astrocytic Ca²⁺ signaling in awake-behaving Alzheimer's disease transgenic mice.

ABSTRACT: Increased astrocytic Ca²⁺ signaling has been shown in Alzheimer's disease mouse models, but to date no reports have characterized behaviorally induced astrocytic Ca²⁺ signaling in such mice. Here, we employ an event-based algorithm to assess astrocytic Ca²⁺ signals in the neocortex of awake-behaving tg-ArcSwe mice and non-transgenic wildtype littermates while monitoring pupil responses and behavior. We demonstrate an attenuated astrocytic Ca²⁺ response to locomotion and an uncoupling of pupil responses and astrocytic Ca²⁺ signaling in 15-month-old plaque-bearing mice. Using the genetically encoded fluorescent norepinephrine sensor GRAB_NE, we demonstrate a reduced norepinephrine signaling during spontaneous running and startl

SUBMITTER: Abjorsbraten KS 

PROVIDER: S-EPMC9352348 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature