Stage-specific transcription activator ESB1 regulates monoallelic antigen expression in Trypanosoma brucei.

ABSTRACT: Variant surface glycoprotein (VSG) coats bloodstream form Trypanosoma brucei parasites, and monoallelic VSG expression underpins the antigenic variation necessary for pathogenicity. One of thousands of VSG genes is transcribed by RNA polymerase I in a singular nuclear structure called the expression site body (ESB), but how monoallelic VSG transcription is achieved remains unclear. Using a localization screen of 153 proteins we found one, ESB-specific protein 1 (ESB1), that localized only to the ESB and is expressed only in VSG-expressing life cycle stages. ESB1 associates with DNA near the active VSG promoter and is necessary for VSG expression, with overexpression activating inactive VSG promoters. Mechanistically, ESB1 is necessary for recruitment of a subset of ESB components, includin

SUBMITTER: Lopez-Escobar L 

PROVIDER: S-EPMC9352583 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature