SmaRT2P: a software for generating and processing smart line recording trajectories for population two-photon calcium imaging.

ABSTRACT: Two-photon fluorescence calcium imaging allows recording the activity of large neural populations with subcellular spatial resolution, but it is typically characterized by low signal-to-noise ratio (SNR) and poor accuracy in detecting single or few action potentials when large number of neurons are imaged. We recently showed that implementing a smart line scanning approach using trajectories that optimally sample the regions of interest increases both the SNR fluorescence signals and the accuracy of single spike detection in population imaging in vivo. However, smart line scanning requires highly specialised software to design recording trajectories, interface with acquisition hardware, and efficiently process acquired data. Furthermore, smart line scanning needs optimized strategies to co

SUBMITTER: Moroni M 

PROVIDER: S-EPMC9352634 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature