Maturation and substrate processing topography of the Plasmodium falciparum invasion/egress protease plasmepsin X.

ABSTRACT: The malaria parasite Plasmodium invades a host erythrocyte, multiplies within a parasitophorous vacuole (PV) and then ruptures the PV and erythrocyte membranes in a process known as egress. Both egress and invasion are controlled by effector proteins discharged from specialized secretory organelles. The aspartic protease plasmepsin X (PM X) regulates activity for many of these effectors, but it is unclear how PM X accesses its diverse substrates that reside in different organelles. PM X also autoprocesses to generate different isoforms. The function of this processing is not understood. We have mapped the self-cleavage sites and have constructed parasites with cleavage site mutations. Surprisingly, a quadruple mutant that remains full-length retains in vitro activity, is trafficked normall

SUBMITTER: Mukherjee S 

PROVIDER: S-EPMC9352755 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature