Synaptotagmin-13 orchestrates pancreatic endocrine cell egression and islet morphogenesis.

ABSTRACT: During pancreas development endocrine cells leave the ductal epithelium to form the islets of Langerhans, but the morphogenetic mechanisms are incompletely understood. Here, we identify the Ca²⁺-independent atypical Synaptotagmin-13 (Syt13) as a key regulator of endocrine cell egression and islet formation. We detect specific upregulation of the Syt13 gene and encoded protein in endocrine precursors and the respective lineage during islet formation. The Syt13 protein is localized to the apical membrane of endocrine precursors and to the front domain of egressing endocrine cells, marking a previously unidentified apical-basal to front-rear repolarization during endocrine precursor cell egression. Knockout of Syt13 impairs endocrine cell egression and skews the α-to-β-cell ratio.

SUBMITTER: Bakhti M 

PROVIDER: S-EPMC9352765 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature