Metagenomic profiling of ocular surface microbiome changes in Demodex blepharitis patients.

ABSTRACT:

Purpose

To compare the ocular surface and meibum microbial communities of humans with Demodex Blepharitis (DB) and healthy controls.

Methods

Conjunctival sac and meibum samples from 25 DB patients and 11 healthy controls were analyzed using metagenomic next-generation sequencing (mNGS).

Results

The alpha-diversity of the conjunctival sac microbiome of the DB group (observed, Chao1, ACE) was lower than that of the control group, whereas all meibum diversity indicators were similar. In conjunctival samples, the relative abundance (RA) of the phylum Proteobacteria was significantly higher (p=0.023), and the RA of both phyla Actinobacteria and Firmicutes was significantly lower (p=0.002, 0.025, respectively) in the DB group than that in the con

SUBMITTER: Fu Y 

PROVIDER: S-EPMC9354880 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature