8-Gene signature related to CD8⁺ T cell infiltration by integrating single-cell and bulk RNA-sequencing in head and neck squamous cell carcinoma.

ABSTRACT: Background: CD8⁺ T cells, a critical component of the tumor immune microenvironment, have become a key target of cancer immunotherapy. Considering the deficiency of robust biomarkers for head and neck squamous cell carcinoma (HNSCC), this study aimed at establishing a molecular signature associated with CD8+T cells infiltration. Methods: Single-cell RNA sequencing data retrieved from the Gene Expression Omnibus (GEO) database was analyzed to obtain the different cell types. Next, the cell proportions were investigated through deconvolution of RNA sequencing in the Cancer Genome Atlas (TCGA) database, and then the immune-related genes (IRGs) were identified by weighted gene co-expression network analysis (WGCNA). LASSO-Cox analysis was employed to establish a gene s

SUBMITTER: Zhang S 

PROVIDER: S-EPMC9355512 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature