Ontology highlight
ABSTRACT:
SUBMITTER: Dickerson JL
PROVIDER: S-EPMC9355893 | biostudies-literature | 2022 Jul
REPOSITORIES: biostudies-literature
Dickerson Joshua L JL Lu Peng-Han PH Hristov Dilyan D Dunin-Borkowski Rafal E RE Russo Christopher J CJ
Ultramicroscopy 20220319
We investigate potential improvements in using electron cryomicroscopy to image thick specimens with high-resolution phase contrast imaging. In particular, using model experiments, electron scattering theory, Monte Carlo and multislice simulations, we determine the potential for improving electron cryomicrographs of proteins within a cell using chromatic aberration (C<sub>c</sub>) correction. We show that inelastically scattered electrons lose a quantifiable amount of spatial coherence as they t ...[more]