Phase contrast imaging with inelastically scattered electrons from any layer of a thick specimen.

ABSTRACT: A controversy exists as to whether the signal in a high resolution phase contrast electron micrograph of a particle in a thick specimen is the same irrespective of the particle's position along the beam axis. Different conceptions of inelastic scattering and its effects on wave interference have led to radically different expectations about the degree of phase contrast vs. depth. Here we examine the information available from bright field phase contrast images of small crystalline particles on the top or bottom of a thick support. The support is an aluminium foil which has strong plasmon resonances that cause a large proportion of the electron beam to lose energy in transit. Phase contrast micrographs of the atomic lattice of two ensembles of platinum particles were measured in an energy l

SUBMITTER: Dickerson JL 

PROVIDER: S-EPMC9355894 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature