Single-cell transcriptome and cell type-specific molecular pathways of human non-alcoholic steatohepatitis.

ABSTRACT: The aim of this study is to characterize cell type-specific transcriptional signatures in non-alcoholic steatohepatitis (NASH) to improve our understanding of the disease. We performed single-cell RNA sequencing on liver biopsies from 10 patients with NASH. We applied weighted gene co-expression network analysis and validated our findings using a publicly available RNA sequencing data set derived from 160 patients with non-alcoholic fatty liver disease (NAFLD) and 24 controls with normal liver histology. Our study provides a comprehensive single-cell analysis of NASH pathology in humans, describing 19,627 single-cell transcriptomes from biopsy-proven NASH patients. Our data suggest that the previous notion of "NASH-associated macrophages" can be explained by an up-regulation of normally ex

SUBMITTER: Fred RG 

PROVIDER: S-EPMC9355943 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature