Engineering rotating apical-out airway organoid for assessing respiratory cilia motility.

ABSTRACT: Motile cilia project from the airway apical surface and directly interface with inhaled external environment. Owing to cilia's nanoscale dimension and high beating frequency, quantitative assessment of their motility remains a sophisticated task. Here we described a robust approach for reproducible engineering of apical-out airway organoid (AOAO) from a defined number of cells. Propelled by exterior-facing cilia beating, the mature AOAO exhibited stable rotational motion when surrounded by Matrigel. We developed a computational framework leveraging computer vision algorithms to quantify AOAO rotation and correlated it with the direct measurement of cilia motility. We further established the feasibility of using AOAO rotation to recapitulate and measure defective cilia motility caused by ch

SUBMITTER: Wijesekara P 

PROVIDER: S-EPMC9356180 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature