Corynoline Alleviates Osteoarthritis Development via the Nrf2/NF-κB Pathway.

ABSTRACT:

Purpose

OA is a multifactorial joint disease in which inflammation plays a substantial role in the destruction of joints. Corynoline (COR), a component of Corydalis bungeana Turcz., has anti-inflammatory effects.

Materials and methods

We evaluated the significance and potential mechanisms of COR in OA development. The viabilities of chondrocytic cells upon COR exposure were assessed by CCK-8 assays. Western blot, qPCR, and ELISA were used to assess extracellular matrix (ECM) degeneration and inflammation. The NF-κB pathway was evaluated by western blot and immunofluorescence (IF). Prediction of the interacting proteins of COR was done by molecular docking, while Nrf2 knockdown by siRNAs was performed to ascertain its significance. Micro-CT, H&E, Safranin O-Fast

SUBMITTER: Li S 

PROVIDER: S-EPMC9356246 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature