Implication of quantifying nitrate utilization and CO₂ assimilation of Brassica napus plantlets in vitro under variable ammonium/nitrate ratios.

ABSTRACT:

Background

Plantlets grown in vitro with a mixed nitrogen source utilize sucrose and CO₂ as carbon sources for growth. However, it is very difficult to obtain the correct utilization proportions of nitrate, ammonium, sucrose and CO₂ for plantlets. Consequently, the biological effect of ammonium/nitrate utilization, the biological effect of sucrose/CO₂ utilization, and the ammonium/nitrate use efficiency for new C input derived from CO₂ assimilation/sucrose utilization are still unclear for plantlets.

Results

The bidirectional stable nitrogen isotope tracer technique quantified the proportions of assimilated nitrate and ammonium in Brassica napus plantlets grown at different ammonium/nitrate ratios. The utilization proportions of su

SUBMITTER: Zhang K 

PROVIDER: S-EPMC9356413 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature