Enhanced activity of hyperthermostable Pyrococcus horikoshii endoglucanase in superbase ionic liquids.

ABSTRACT:

Objectives

Ionic liquids (ILs) that dissolve biomass are harmful to the enzymes that degrade lignocellulose. Enzyme hyperthermostability promotes a tolerance to ILs. Therefore, the limits of hyperthemophilic Pyrococcus horikoschii endoglucanase (PhEG) to tolerate 11 superbase ILs were explored.

Results

PhEG was found to be most tolerant to 1-ethyl-3-methylimidazolium acetate ([EMIM]OAc) in soluble 1% carboxymethylcellulose (CMC) and insoluble 1% Avicel substrates. At 35% concentration, this IL caused an increase in enzyme activity (up to 1.5-fold) with CMC. Several ILs were more enzyme inhibiting with insoluble Avicel than with soluble CMC. K_m increased greatly in the presence ILs, indicating significant competitive inhibition. Increased hydrophobicity of the IL c

SUBMITTER: Hebal H 

PROVIDER: S-EPMC9356960 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature