Selective isolation of extracellular vesicles from minimally processed human plasma as a translational strategy for liquid biopsies.

ABSTRACT:

Background

Intercellular communication is mediated by extracellular vesicles (EVs), as they enclose selectively packaged biomolecules that can be horizontally transferred from donor to recipient cells. Because all cells constantly generate and recycle EVs, they provide accurate timed snapshots of individual pathophysiological status. Since blood plasma circulates through the whole body, it is often the biofluid of choice for biomarker detection in EVs. Blood collection is easy and minimally invasive, yet reproducible procedures to obtain pure EV samples from circulating biofluids are still lacking. Here, we addressed central aspects of EV immunoaffinity isolation from simple and complex matrices, such as plasma.

Methods

Cell-generated EV spike-in models were isolated and pur

SUBMITTER: Fortunato D 

PROVIDER: S-EPMC9357340 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature