An allied reprogramming, selection, expansion and differentiation platform for creating hiPSC on microcarriers

ABSTRACT: Abstract

Objectives

Induced pluripotent stem cells (iPSCs) generated by monolayer cultures is plagued by low efficiencies, high levels of manipulation and operator unpredictability. We have developed a platform, reprogramming, expansion, and differentiation on Microcarriers, to solve these challenges.

Materials and Methods

Five sources of human somatic cells were reprogrammed, selected, expanded and differentiated in microcarriers suspension cultures.

Results

Improvement of transduction efficiencies up to 2 times was observed. Accelerated reprogramming in microcarrier cultures was 7 days faster than monolayer, providing between 30 and 50‐fold more clones to choose from fibroblasts, peripheral blood mononuclear cells, T cells and CD34+ stem cells. This was observe

SUBMITTER: Lam A 

PROVIDER: S-EPMC9357361 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature