CircPCBP2 promotes the stemness and chemoresistance of DLBCL via targeting miR-33a/b to disinhibit PD-L1.

ABSTRACT: Diffuse large B-cell lymphoma (DLBCL) is the most common lymphoid malignancy with a high relapse rate of up to 40%. The prognosis of the disease needs improvement and requires a understanding of its molecular mechanism. We investigated the mechanisms of DLBCL development and its sensitivity to chemotherapy by focusing on circPCBP2/miR-33a/b/PD-L1 axis. Human DLBCL specimens and cultured cancer cell lines were used. Features of circPCBP2 were systematically characterized through Sanger sequencing, Actinomycin D, RNase R treatment, and FISH. The expression levels of circPCBP2, miR-33a/b, PD-L1, stemness-related markers, ERK/AKT and JAK2/STAT3 signaling were measured using qRT-PCR, western blotting, and immunohistochemistry. Stemness of DLBCL cells was assessed through spheroid formation assa

SUBMITTER: Dong L 

PROVIDER: S-EPMC9357607 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature