Label-free multiphoton excitation imaging as a promising diagnostic tool for breast cancer.

ABSTRACT: Histopathological diagnosis is the ultimate method of attaining the final diagnosis; however, the observation range is limited to the two-dimensional plane, and it requires thin slicing of the tissue, which limits diagnostic information. To seek solutions for these problems, we proposed a novel imaging-based histopathological examination. We used the multiphoton excitation microscopy (MPM) technique to establish a method for visualizing unfixed/unstained human breast tissues. Under near-infrared ray excitation, fresh human breast tissues emitted fluorescent signals with three major peaks, which enabled visualizing the breast tissue morphology without any fixation or dye staining. Our study using human breast tissue samples from 32 patients indicated that experienced pathologists can estima

SUBMITTER: Matsui T 

PROVIDER: S-EPMC9357641 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature