Construction of immunotherapy-related prognostic gene signature and small molecule drug prediction for cutaneous melanoma.

ABSTRACT:

Background

Cutaneous melanoma (CM), a kind of skin cancer with a high rate of advanced mortality, exhibits a wide variety of driver and transmitter gene alterations in the immunological tumor microenvironment (TME) associated with tumor cell survival and proliferation.

Methods

We analyzed the immunological infiltration of TME cells in normal and malignant tissues using 469 CM and 556 normal skin samples. We used a single sample gene set enrichment assay (ssGSEA) to quantify the relative abundance of 28 cells, then used the LASSO COX regression model to develop a riskScore prognostic model, followed by a small molecule drug screening and molecular docking validation, which was then validated using qRT-PCR and IHC.

Results

We developed a prognosis model around seven ess

SUBMITTER: Xing J 

PROVIDER: S-EPMC9358033 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature