An open source 16-channel fluidics system for automating sequential fluorescent in situ hybridization (FISH)-based imaging.

ABSTRACT: Fluorescent in situ hybridization (FISH) can provide spatial information about DNA/RNA targets in fixed cells and tissues. However, the workflows of multiplexed FISH-based imaging that use sequential rounds of hybridization quickly become laborious as the number of rounds increases because of liquid handling demands. Here, we present an open-source and low-cost fluidics system that is purpose built for automating the workflows of sequential FISH-based imaging. Our system features a fluidics module with 16 addressable channels in which flow is positive pressure-driven and switched on/off by solenoid valves in order to transfer FISH reagents to the sample. Our system also includes a controller with a main printed circuit board that can control up to 120 solenoid valves and allows user

SUBMITTER: Deng Z 

PROVIDER: S-EPMC9358477 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature