Native metabolomics identifies the rivulariapeptolide family of protease inhibitors.

ABSTRACT: The identity and biological activity of most metabolites still remain unknown. A bottleneck in the exploration of metabolite structures and pharmaceutical activities is the compound purification needed for bioactivity assignments and downstream structure elucidation. To enable bioactivity-focused compound identification from complex mixtures, we develop a scalable native metabolomics approach that integrates non-targeted liquid chromatography tandem mass spectrometry and detection of protein binding via native mass spectrometry. A native metabolomics screen for protease inhibitors from an environmental cyanobacteria community reveals 30 chymotrypsin-binding cyclodepsipeptides. Guided by the native metabolomics results, we select and purify five of these compounds for full structure elucida

SUBMITTER: Reher R 

PROVIDER: S-EPMC9358669 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature