Cryptosporidium diagnosis in different groups of children and characterization of parasite species.

ABSTRACT:

Background

Microscopy and enzyme-linked immunosorbent assay (ELISA) are routinely used for Cryptosporidium diagnosis, without differentiating the parasite species.

Methods

Children's feces were analyzed by modified Ziehl-Neelsen (mZN) and ELISA for Cryptosporidium diagnosis and by polymerase chain reaction-restriction fragment length polymorphism for species identification.

Results

Cryptosporidium frequency was 2.6%. The sensitivity and specificity of ELISA were 85.7% and 99.7%, respectively, with excellent concordance with mZN (kappa=0.854). Parasite species were characterized as Cryptosporidium hominis (78.3%), Cryptosporidium felis (17.4%), and Cryptosporidium parvum (4.3%).

Conclusions

Coproantigen ELISA is as efficient as mZN for Cryptosporidium diagnosis.

SUBMITTER: Pacheco FTF 

PROVIDER: S-EPMC9359344 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature