Transcriptional profiling demonstrates altered characteristics of CD8⁺ cytotoxic T-cells and regulatory T-cells in TP53-mutated acute myeloid leukemia.

ABSTRACT:

Background

Acute myeloid leukemia (AML) patients have limited effect from T-cell-based therapies, such as PD-1 and CTLA-4 blockade. However, recent data indicate that AML patients with TP53 mutation have higher immune infiltration and other immunomodulatory therapies could thus potentially be effective. Here, we performed the transcriptional analysis of distinct T-cell subpopulations from TP53-mutated AML to identify gene expression signatures suggestive of altered functional properties.

Methods

CD8⁺ cytotoxic T lymphocytes (CTLs), conventional helper T cells (Th), and regulatory T cells (Tregs) were sorted from peripheral blood of AML patients with TP53 mutation (n = 5) and healthy donors (n = 3), using FACS, and the different subpopulations were subsequently sub

SUBMITTER: Abolhalaj M 

PROVIDER: S-EPMC9359873 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature