Photonic crystal enhanced fluorescence emission and blinking suppression for single quantum dot digital resolution biosensing.

ABSTRACT: While nanoscale quantum emitters are effective tags for measuring biomolecular interactions, their utilities for applications that demand single-unit observations are limited by the requirements for large numerical aperture (NA) objectives, fluorescence intermittency, and poor photon collection efficiency resulted from omnidirectional emission. Here, we report a nearly 3000-fold signal enhancement achieved through multiplicative effects of enhanced excitation, highly directional extraction, quantum efficiency improvement, and blinking suppression through a photonic crystal (PC) surface. The approach achieves single quantum dot (QD) sensitivity with high signal-to-noise ratio, even when using a low-NA lens and an inexpensive optical setup. The blinking suppression capability of the PC impro

SUBMITTER: Xiong Y 

PROVIDER: S-EPMC9360002 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature