Dataset Information

MiRNA-23b-5p affects the proliferation, migration and invasion of osteosarcoma by targeting TMEM127.

ABSTRACT:

Background

Osteosarcoma (OS) has become one of the highest mortality cancers in the world due to its late diagnosis, rapid metastasis and rapid recurrence. MicroRNAs can regulate a variety of signaling pathwas involved in cancer development, such as cell proliferation, apoptosis and migration.

Objective

In this study, we studied the biological effects and molecular regulation of mir-23b-5p on human osteosarcoma cells.

Methods

The proliferation of mir-23b-5p in osteosarcoma was measured by CCK8 method and EDU method. In addition, the target population was screened through the database, and the luciferase reporter gene was used to determine the association between miRNA and target gene TMEM127. We verified this result by Western blot.

Results

We found that mir-23b-5p promotes the progression of osteosarcoma by regulating TMEM127.

Conclusions

The results of this study show that mir-23b-5p affects the proliferation, metastasis and invasion of OS by targeting TMEM127.

SUBMITTER: Zu D

PROVIDER: S-EPMC9360392 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Publications

miRNA-23b-5p affects the proliferation, migration and invasion of osteosarcoma by targeting TMEM127.

Zu Dan D Dong Qi Q Yao Jun J Chen Sunfang S Fang Bin B Ma Jun J Wu Bing B

Discover oncology 20220808 1

<h4>Background</h4>Osteosarcoma (OS) has become one of the highest mortality cancers in the world due to its late diagnosis, rapid metastasis and rapid recurrence. MicroRNAs can regulate a variety of signaling pathwas involved in cancer development, such as cell proliferation, apoptosis and migration.<h4>Objective</h4>In this study, we studied the biological effects and molecular regulation of mir-23b-5p on human osteosarcoma cells.<h4>Methods</h4>The proliferation of mir-23b-5p in osteosarcoma ...[more]

PMID: 35941331

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Project description:BackgroundStudies of aberrantly expressed circular RNAs (circRNAs) can provide insights into the molecular mechanisms of osteosarcoma (OS). However, the role of circ_0001174 in OS progression remains unknown. This study is aimed to identify differentially expressed circRNAs and messenger RNAs (mRNAs) in patients with OS and to investigate potential regulatory ways of circ_0001174.MethodsHigh-throughput sequencing was performed to screen aberrantly expressed circRNAs and mRNAs between tumor and paracancerous tissues from patients with OS. Several bioinformatics tools were used to analyze the functions and pathways of the differentially expressed genes between the tissues. Cell counting kit-8, cell migration and invasion assays were performed to evaluate the functions of the critical circRNAs. RNA interference experiments, quantitative real-time polymerase chain reaction (RT-qPCR) and western blotting were used to explore the relationship between miR-186-5p and circ_0001174 or metastasis-associated in colon cancer 1 (MACC1).ResultsCompared with the paracancerous tissues, 109 circRNAs and 1264 mRNAs were differentially expressed in the OS tissues, including 88 circRNAs and 707 mRNAs that were upregulated and 21 circRNAs and 557 mRNAs that were downregulated. The expression of four upregulated and four downregulated circRNAs was validated using RT-qPCR; the results were consistent with the sequencing data, and circ_0001174 was found to be significantly upregulated in 16 pairs of OS tissues and OS cell lines (fold change > 2.0, P value < 0.05). Knockdown of circ_0001174 inhibited the proliferation, migration, and invasion of OS cells. Additionally, circ_0001174 directly and negatively modulated the expression of miR-186-5p and positively regulated the expression of MACC1.ConclusionsAbnormally high expression of circ_0001174 may promote the proliferation, migration, and invasion of OS cells through up-regulating MACC1 by sponging miR-186-5p. These results provide insight into therapeutic targets for preventing and treating OS.