MiRNA-23b-5p affects the proliferation, migration and invasion of osteosarcoma by targeting TMEM127.

ABSTRACT:

Background

Osteosarcoma (OS) has become one of the highest mortality cancers in the world due to its late diagnosis, rapid metastasis and rapid recurrence. MicroRNAs can regulate a variety of signaling pathwas involved in cancer development, such as cell proliferation, apoptosis and migration.

Objective

In this study, we studied the biological effects and molecular regulation of mir-23b-5p on human osteosarcoma cells.

Methods

The proliferation of mir-23b-5p in osteosarcoma was measured by CCK8 method and EDU method. In addition, the target population was screened through the database, and the luciferase reporter gene was used to determine the association between miRNA and target gene TMEM127. We verified this result by Western blot.

Results

We found that mir-23

SUBMITTER: Zu D 

PROVIDER: S-EPMC9360392 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature