Subcellular analysis of nuclear and cytoplasmic redox indices differentiates breast cancer cell subtypes better than nuclear-to-cytoplasmic area ratio.

ABSTRACT:

Significance

Stratification of malignancy is valuable for cancer treatment. Both optical redox imaging (ORI) indices and nuclear-to-cytoplasmic volume/area ratio (N:C ratio) have been investigated to differentiate between cancers with varying aggressiveness, but these two methods have not been directly compared. The redox status in the cell nucleus has not been studied by ORI, and it remains unknown whether nuclear ORI indices add new biological information.

Aim

We sought to compare the capacity of whole-cell and subcellular ORI indices and N:C ratio to differentiate between breast cancer subtypes with varying aggressiveness and between mitotic and nonmitotic cells.

Approach

ORI indices for whole cell, cytoplasm, and nucleus as well as the N:C area ratio were generate

SUBMITTER: Jacob A 

PROVIDER: S-EPMC9360498 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature