Efficient fluorescence-based localization technique for real-time tracking endophytes route in host-plants colonization.

ABSTRACT: Bacterial isolates that enhance plant growth and suppress plant pathogens growth are essential tools for reducing pesticide applications in plant production systems. The objectives of this study were to develop a reliable fluorescence-based technique for labeling bacterial isolates selected as biological control agents (BCAs) to allow their direct tracking in the host-plant interactions, understand the BCA localization within their host plants, and the route of plant colonization. Objectives were achieved by developing competent BCAs transformed with two plasmids, pBSU101 and pANIC-10A, containing reporter genes eGFP and pporRFP, respectively. Our results revealed that the plasmid-mediated transformation efficiencies of antibiotic-resistant competent BCAs identified as PSL, I

SUBMITTER: Ondzighi-Assoume CA 

PROVIDER: S-EPMC9360559 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature