MiR-365-3p is a negative regulator in IL-17-mediated asthmatic inflammation.

ABSTRACT:

Background

Interleukin-17, the major proinflammatory cytokine secreted by Th17 cells, makes essential contribution to pathogenesis of severe asthma, while the detailed mechanisms, especially the involvement of microRNAs which are also important participants in asthma progression, remains largely unclear.

Methods

In this study, we established a house dust mite (HDM) extract-induced murine asthmatic models and the miRNA expression in the lung tissues of mice were profiled by miRNA microarray assay. The effect of miR-365-3p on IL-17-mediated inflammation was examined by qRT-PCR and immunoblotting analysis. The involvement of ARRB2 as target gene of miR-365-3p was verified by overexpression or RNA interference.

Results

HDM extract-induced asthmatic inflammation was proved

SUBMITTER: Wang W 

PROVIDER: S-EPMC9361323 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature