Uncovering Hidden Members and Functions of the Soil Microbiome Using De Novo Metaproteomics.

ABSTRACT: Metaproteomics has been increasingly utilized for high-throughput characterization of proteins in complex environments and has been demonstrated to provide insights into microbial composition and functional roles. However, significant challenges remain in metaproteomic data analysis, including creation of a sample-specific protein sequence database. A well-matched database is a requirement for successful metaproteomics analysis, and the accuracy and sensitivity of PSM identification algorithms suffer when the database is incomplete or contains extraneous sequences. When matched DNA sequencing data of the sample is unavailable or incomplete, creating the proteome database that accurately represents the organisms in the sample is a challenge. Here, we leverage a de novo peptide sequen

SUBMITTER: Lee JY 

PROVIDER: S-EPMC9361346 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature