SimPLIT: Simplified Sample Preparation for Large-Scale Isobaric Tagging Proteomics.

ABSTRACT: Large scale proteomic profiling of cell lines can reveal molecular signatures attributed to variable genotypes or induced perturbations, enabling proteogenomic associations and elucidation of pharmacological mechanisms of action. Although isobaric labeling has increased the throughput of proteomic analysis, the commonly used sample preparation workflows often require time-consuming steps and costly consumables, limiting their suitability for large scale studies. Here, we present a simplified and cost-effective one-pot reaction workflow in a 96-well plate format (SimPLIT) that minimizes processing steps and demonstrates improved reproducibility compared to alternative approaches. The workflow is based on a sodium deoxycholate lysis buffer and a single detergent cleanup step after peptide la

SUBMITTER: Sialana FJ 

PROVIDER: S-EPMC9361352 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature