Balanced mitochondrial and cytosolic translatomes underlie the biogenesis of human respiratory complexes.

ABSTRACT:

Background

Oxidative phosphorylation (OXPHOS) complexes consist of nuclear and mitochondrial DNA-encoded subunits. Their biogenesis requires cross-compartment gene regulation to mitigate the accumulation of disproportionate subunits. To determine how human cells coordinate mitochondrial and nuclear gene expression processes, we tailored ribosome profiling for the unique features of the human mitoribosome.

Results

We resolve features of mitochondrial translation initiation and identify a small ORF in the 3' UTR of MT-ND5. Analysis of ribosome footprints in five cell types reveals that average mitochondrial synthesis levels correspond precisely to cytosolic levels across OXPHOS complexes, and these average rates reflect the relative abundances of the complexes. Balanced mitoch

SUBMITTER: Soto I 

PROVIDER: S-EPMC9361522 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature