HPV genotyping by L1 amplicon sequencing of archived invasive cervical cancer samples: a pilot study.

ABSTRACT:

Background

Human papillomavirus (HPV) is the primary cause of invasive cervical cancer (ICC). The prevalence of various HPV genotypes, ranging from oncogenically low- to high-risk, may be influenced by geographic and demographic factors, which could have critical implications for the screening and prevention of HPV infection and ICC incidence. However, many technical factors may influence the identification of high-risk genotypes associated with ICC in different populations.

Methods

We used high-throughput sequencing of a single amplicon within the HPV L1 gene to assess the influence of patient age, race/ethnicity, histological subtype, sample type, collection date, experimental factors, and computational parameters on the prevalence of HPV genotypes detected in archived DNA

SUBMITTER: Warden CD 

PROVIDER: S-EPMC9361560 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature