Blockade of exosome generation by GW4869 inhibits the education of M2 macrophages in prostate cancer.

ABSTRACT:

Background

Tumor-associated macrophages are considered to be a major contributor affecting the development of tumors. Recently, numerous studies have shown that tumor cells were able to educate their microenvironment by delivering a significant amount of exosomes, however, the mechanism that exosomes from PCa cells work in macrophage polarization remains obscure. Therefore, we sought to determine whether blockade of exosome generation by GW4869, an inhibitor of exosome biogenesis, would impede macrophages from differentiating into M2 cells.

Results

In this study, we first obtained exosomes from the supernatant media of PCa cells cultured with exosome-free serum using the Magcapture™ Exosome Isolation Kit PS, and then investigated their effects on macrophages. Our data confir

SUBMITTER: Peng Y 

PROVIDER: S-EPMC9361607 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature