A single cell survey of the microbial impacts on the mouse small intestinal epithelium.

ABSTRACT: The small intestinal epithelial barrier inputs signals from the gut microbiota in order to balance physiological inflammation and tolerance, and to promote homeostasis. Understanding the dynamic relationship between microbes and intestinal epithelial cells has been a challenge given the cellular heterogeneity associated with the epithelium and the inherent difficulty of isolating and identifying individual cell types. Here, we used single-cell RNA sequencing of small intestinal epithelial cells from germ-free and specific pathogen-free mice to study microbe-epithelium crosstalk at the single-cell resolution. The presence of microbiota did not impact overall cellular composition of the epithelium, except for an increase in Paneth cell numbers. Contrary to expectations, pattern recognition r

SUBMITTER: Tsang DKL 

PROVIDER: S-EPMC9361762 | biostudies-literature | 2022 Jan-Dec

REPOSITORIES: biostudies-literature