Characterization of monoclonal antibodies that specifically differentiate field isolates from vaccine strains of classical swine fever virus.

ABSTRACT: Classical swine fever virus (CSFV) is a major animal pathogen threatening the global pork industry. To date, numerous anti-CSFV monoclonal antibodies (mAbs) and their recognizing epitopes have been reported. However, few mAbs were systematically characterized for the capacity to differentiate field CSFV isolates from CSF vaccine strains, and the molecular basis associated with antigenic differences between vaccines and field isolates is still largely unknown. In the present study, recombinant CSFV structural glycoproteins E2 of both virulent and vaccine strains and E^rns of vaccine strain were expressed using eukaryotic cells and murine mAbs generated against E2 and E^rns. After serial screening and cloning of the hybridomas, the viral spectra of mAbs were respectively

SUBMITTER: Mi S 

PROVIDER: S-EPMC9361847 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature