Single-cell transcriptomics reveals shared immunosuppressive landscapes of mouse and human neuroblastoma.

ABSTRACT:

Background

High-risk neuroblastoma is a pediatric cancer with still a dismal prognosis, despite multimodal and intensive therapies. Tumor microenvironment represents a key component of the tumor ecosystem the complexity of which has to be accurately understood to define selective targeting opportunities, including immune-based therapies.

Methods

We combined various approaches including single-cell transcriptomics to dissect the tumor microenvironment of both a transgenic mouse neuroblastoma model and a cohort of 10 biopsies from neuroblastoma patients, either at diagnosis or at relapse. Features of related cells were validated by multicolor flow cytometry and functional assays.

Results

We show that the immune microenvironment of MYCN-driven mouse neuroblastoma is char

SUBMITTER: Costa A 

PROVIDER: S-EPMC9362821 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature