Shotgun proteomic profiling of dormant, 'non-culturable' Mycobacterium tuberculosis.

ABSTRACT: Dormant cells of Mycobacterium tuberculosis, in addition to low metabolic activity and a high level of drug resistance, are characterized by 'non-culturability'-a specific reversible state of the inability of the cells to grow on solid media. The biochemical characterization of this physiological state of the pathogen is only superficial, pending clarification of the metabolic processes that may exist in such cells. In this study, applying LC-MS proteomic profiling, we report the analysis of proteins accumulated in dormant, 'non-culturable' M. tuberculosis cells in an in vitro model of self-acidification of mycobacteria in the post-stationary phase, simulating the in vivo persistence conditions-the raw data are available via ProteomeXchange with identifier PXD028849. This approach revealed

SUBMITTER: Nikitushkin V 

PROVIDER: S-EPMC9362914 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature