Isolation and Identification of Free Living Amoeba from Patients and Contact Lens Users in Iran.

ABSTRACT:

Background

Free-living amoebae (FLA) such as Acanthamoeba spp., are considered as opportunistic and pathogenic protozoans. Acanthamoeba granulomatous encephalitis (AGE) is a serious threat for immunodeficient patients and Acanthamoeba keratitis (AK) for contact lens users. We aimed to identify the presence of free living amoebae in nasal swabs of patients and contact lens users in Qazvin, Iran.

Methods

During 2019, 251 nasal and oral swabs (including the pharynx and mouth) were collected from patients with diabetes, AIDS and those under periodic dialysis in Qazvin, Iran. In addition, 27 soft contact lenses were collected from the participants. Following DNA extraction, PCR and sequencing were conducted to identify the genotypes of the amoeba. Phylogeneti

SUBMITTER: Jalilehvand M 

PROVIDER: S-EPMC9363255 | biostudies-literature | 2022 Apr-Jun

REPOSITORIES: biostudies-literature