Phenotypic Epithelial Changes in Laryngotracheal Stenosis.

ABSTRACT:

Objective

Characterize and quantify epithelium in multiple etiologies of laryngotracheal stenosis (LTS) to better understand its role in pathogenesis.

Study design

Controlled in vitro cohort study.

Methods

Endoscopic brush biopsy samples of both normal (non-scar) and scar were obtained in four patients with idiopathic subglottic stenosis (iSGS) and four patients with iatrogenic LTS (iLTS). mRNA expression of basal, ciliary, and secretory cell markers were evaluated using quantitative PCR. Cricotracheal resection tissue samples (n = 5 per group) were also collected, analyzed using quantitative immunohistochemistry, and compared with rapid autopsy tracheal samples.

Results

Both iSGS and iLTS-scar epithelium had reduced epithelial thickness compared with non-scar

SUBMITTER: Lina IA 

PROVIDER: S-EPMC9363526 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature