Branched Chain Amino Acids Promote ATP Production Via Translocation of Glucose Transporters.

ABSTRACT:

Purpose

We have previously shown that maintenance of ATP levels is a promising strategy for preventing neuronal cell death, and that branched chain amino acids (BCAAs) enhanced cellular ATP levels in cultured cells and antagonized cell death. BCAAs attenuated photoreceptor degeneration and retinal ganglion cell death in rodent models of retinal degeneration or glaucoma. This study aimed to elucidate the mechanisms through which BCAAs enhance ATP production.

Methods

Intracellular ATP concentration was measured in HeLa cells under glycolysis and citric acid cycle inhibited conditions. Next, glucose uptake was quantified in HeLa cells and in 661W retinal photoreceptor-derived cells under glycolysis inhibition, endoplasmic reticulum stress, and glucose transporters (GLUTs) inhib

SUBMITTER: Iwai S 

PROVIDER: S-EPMC9363681 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature