Lupeol and pristimerin do not inhibit activation of the human sperm CatSper Ca(2+)-channel.

ABSTRACT: Opposing findings have been published on the regulation of the sperm-specific Ca ²⁺ channel CatSper (cation channel of sperm) in human sperm cells by the plant triterpenoids lupeol and pristimerin. While the original study on this topic found these triterpenoids to act as potent inhibitors of human CatSper, subsequent studies have failed to replicate such an inhibitory effect. It has been suggested that these issues could in part be due to purity issues and/or batch variation between the plant-derived extracts of lupeol and pristimerin obtained for the studies. The aim of this study was to elucidate this controversy by investigating the batches of lupeol and pristimerin used in our previous study with state-of-the-art ¹H-, ¹³C- and 2D-nuclear magnetic reson

SUBMITTER: Rehfeld A 

PROVIDER: S-EPMC9363974 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature